李琼,郭晏强,马西平

• 1:平顶山市疾病预防控制中心(平顶山市卫生监督所)

摘要(Abstract)：

目的 分析2022—2024年平顶山市食品安全风险监测中分离的17株单核细胞增生李斯特菌的药物敏感性、分子分型及耐药基因特征，为地方食品安全监测提供基础资料。方法 采用微量肉汤稀释法测定15种抗菌药物最低抑菌浓度（MIC）；依据CLSI M45对有对应临床折点的药物进行判读，对无明确折点药物仅描述MIC分布；基于全基因组测序结果开展MLST、谱系判定、耐药基因筛查及cgMLST分析。结果 17株菌主要来源于肉及肉制品（8株）和水产品（7株），另有预包装冷藏即食食品和速冻菜肴制品各1株。共检出7种明确ST型，以ST87、ST619和ST121为主。青霉素、四环素、万古霉素和复方新诺明均为敏感；红霉素13株敏感、4株中介，未检出红霉素耐药株。部分无明确折点药物的MIC分布存在株间差异。cgMLST可将菌株分为3个主要簇，并识别LM9/LM10、LM12/LM14等近缘小簇。多数耐药相关基因为全部菌株共有，仅LM12检出ermC，且其红霉素和克林霉素MIC较高。结论 本研究菌株分子型别多样，不同抗菌药物MIC分布存在差异，cgMLST较MLST具有更高分辨率，可为本地区食品安全风险监测和溯源分析提供基础资料。

关键词(KeyWords)： 单核细胞增生李斯特菌;食品安全风险监测;最低抑菌浓度;多位点序列分型;核心基因组多位点序列分型;耐药基因

Abstract:

Keywords:

基金项目(Foundation): 河南省医学科技攻关计划项目（LHGJ20250138）

作者(Author): 李琼,郭晏强,马西平

DOI: 10.13515/j.cnki.hnjpm.1006-8414.2026.09.013

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